The ethnomedicinal use of Pyrenacantha staudtii leaf as remedy against threatened abortion was evaluated through activity guided fractionation techniques on rat isolated uterus in our earlier study.One of the biologically active fraction was subjeccetd to reversed phase lobar column C-18 chromatography with different solvent systems and other chromatographic methods. The structure of the pure compound isolated was characterized by spectroscopic techniques such as IR, NMR, MS and elemental analysis.

3-carbomethoxypyridine was isolated and characterized from the leaves of Pyrenacantha staudtii Hutch and Dalz Icacinaceae, in our earlier study and was found to possess inhibitory activity on the isolated rat uterus. In order to study the structure activity relationship, derivatives of CMP were obtained synthetically, purified  and characterized by spectroscopic techniques such as infra red (IR), nuclea magnetic resonance (H and  C  NMR) and mass spectrometry (MS).